The chemical constitutes of the EtOAc fraction from Lethariella zahlbruckneri (Dr.) Krog.,collected from Qinghai Province,were studied.Five compounds were isolated by repeated gel column chromatography,semi-prepared HPLC and re-crystallization.Their structures were characterized by ¹H-NMR and ¹³C-NMR.Compound 5 is vermicularin,belongs to depsides;Compound 1 is Methyl haematommate;Compound 2 is methyl β-orcinolcarboxylate;Compound 3 is methyl orsellinate;Compound 4 is ethyl orsellinate;The above four compounds all belong to cleavage products of depsides and depsidones.Compounds 1 , 3 , 4 , 5 were isolated from L.zahlbruckneri for the first time.