Primers were designed based on homologous sequences of previously reported Arabidopsis thaliana and Brassica napus L..BcHSP70-1 was isolated from Brassica campestris ssp.chinensis.The physical properties of BcHSP70-1 gene,together with subcellular localization and transmembrane domain were analyzed with bioinformatics.The results showed that:BcHSP70-1 contains 1 950 bp (ORF) nucleotides and encodes 649 amino acids,which is hydrophilic protein and localized in the cytoplasm.BcHSP70-1 has one intron and two exons,the intron is 324 bp.Comparison of orthologous exons and introns of HSP70-1 from different species showed that introns have more significant differences.Using real-time quantitative PCR analysis showed that BcHSP70-1 expressed differently among the different heat resistant cultivars.The heat sensitive cultivar (NHCC002) had no constitutive expression of the gene.The qRT-PCR results showed that,compared to 38 ℃ high temperature stress,BcHSP70-1 had higher expressed levels at 4 ℃ low temperature stress in non-heading Chinese cabbage.