Using RT-PCR and RACE methods,we cloned a SPS gene from a medicinally plant Lycium barbarum L.,named as LbSPS (GenBank number:KC834608).Sequence analyses showed that the full length cDNA of LbSPS was 3 677 bp and the 3 165 bp ORF encoded 1 033 amino acids with a molecular weight of 118.457 5 kD and an isoelectric point of 6.05.Phylogenetic analysis showed that the amino acid sequences of LbSPS were 66%～98% identical to the sequences of melon,tomato,potato,etc.qRT-PCR analysis showed that the LbSPS gene was highly expressed in flower.The expression level reached the lowest in leaf.Molecular cloning of LbSPS will be useful for further functional elucidation of the gene involving in the development,stress response in L.barbarum L..