The allelic compositions of high-molecular-weight glutenin subunit (HMW-GS) and the alleles on Glu-A3 and Glu-B3 loci of low-molecular-weight glutenin subunit(LMW-GS) in the wheat cultivars and lines in Southern Shaanxi were identified by SDS-PAGE and STS molecular markers,respectively;and the partial allelic results of HMW-GS from SDS-PAGE were validated by STS specific marker.The results showed that:(1)There were nine types of allelic variations of HMW-GS in 64 cultivated materials in Southern Shaanxi.The frequencies of the Null and 1 alleles at Glu-A1 locus were 53.12% and 46.88%,respectively;the alleles of 7+8,7+9,14+15 and 17+18 at Glu-B1 locus are 26.56%,48.44%,21.88% and 3.13%,respectively;the alleles of 2 +12,5+10 and 4+12 at Glu-D1 locus were 71.88%,15.63% and 12.49%,respectively and the 9 HMW-GS alleles formed 17 types of permutations in which “1,7+9,2+12” and “null,7+9,2+12” were the major styles.(2)The frequencies of the allelic variations of Glu-A3a,Glu-A3c and Glu-A3d at Glu-A3 locus of LMW-GS are 10.94%,62.50% and 26.56%,respectively;and the alleles of Glu-B3a,Glu-B3b,Glu-B3d,Glu-B3e,Glu-B3f,Glu-B3g,Glu-B3i and Glu-B3j at Glu-B3 locus of LMW-GS are 6.25%,4.69%,29.69%,1.56%,3.13%,18.75%,4.69% and 31.25%,respectively.(3)The validated results from 2 STS specific markers for the allelic compositions of HMW-GS by SDS-PAGE could demonstrate that STS specific markers might efficiently overcome the misreading for the alleles of 7 and 7^*,8 and 8^* and 2 and 2^*.