Genetic diversities of 5 wild soybean populations from Guangdong were examined by SSR.The results showed that:(1)There were 263 alleles at 60 SSR loci,varying from 2 to 10 with an average of 4.38 alleles for each locus.Lianzhou and Nanxiong populations had higher unique alleles,each with 19 and 16.Shannon diversity indexes varied from 0.162 to 2.174,while the expected heterozygosity (H_e) ranged from 0.073 to 0.899.(2)Lianzhou populations had the highest genetic diversity with Shannon diversity index 0.811 among 5 wild soybean populations in Guangdong,while Renhua population had the lowest genetic diversity with Shannon diversity index 0.113.AMOVA analysis results indicated that there is 51% variance among the populations and 49% in populations.Renhua population（RH） had lower gene flow with the other populations.According to genetic distance the 5 populations were divided into three groups:Lianzhou and Liannan as a group,Ruyuan and Nanxiong as a group,Renhua only for a group.The results indicated that there were different levels in genetic diversity among populations and within population,and Lianzhou and Ruyuan populations had higher genetic diversity than others.