With ‘ML7113’ wheat seedlings as the materials,artificial simulation of He-Ne laser（5 mJ·s^-1·mm^-2）,enhanced UV-B(10.8 kJ·m^-2·d^-1) radiation and combined radiation processing.Using chlorophyll fluorescence spectrometer,coomassie brilliant blue G-250 dyeing method and PCR technique,we studied the changes of chlorophyll fluorescence characteristics,content of rubisco activase,changes of gene expression and gene sequence on seven day age of wheat seedling.The results show that:(1)Compared with the control group,after enhanced UV-B radiation,wheat seedling chlorophyll fluorescence characteristics is abate.Rubisco activase content and its gene expression quantity decreased.But low doses of He-Ne laser irradiation can repair the damage on wheat seedling chlorophyll fluorescence characteristics which caused by after UV-B radiation and make rubisco activase content and its gene expression increased.(2)Compared with the control group,after He-Ne laser and enhanced UV-B radiation and combined irradiation gene sequences were presented with two identical point mutations,but did not cause the change of amino acid sequence.Visible,low dose He-Ne laser irradiation can be repaired by UV-B radiation on chlorophyll fluorescence activity of wheat seedlings to a certain extent and make the rubisco activase content and its lower amount of gene expression.Speculate that the influence of He-Ne laser and enhanced UV-B radiation on wheat seedling rubisco activase due to the change of transcriptional.So that the wheat photosynthetic capacity was changed accordingly.