The fresh pollens of 6 a ‘Jinhua’ pecan (Carya illinoinensis) tree were used in this study.The focus was to investigate the regulatory role of sucrose,boric acid,Ca²⁺,rehydration time and temperature in pecan pollen germination and pollen tube growth by in vitro culture.And the results showed that:(1)The rehydration for 4 h before the test is necessary which could improve pollen germination significantly;(2)Sucrose, boric acid and Ca²⁺ could stimulate pollen germination and tube growth,but this function would reverse if overrun certain concentration;(3)The research suggested that the optimum culture medium consist of 20% sucrose,0.02%~0.03% H₃BO₃ and 0.05% Ca(NO₃)₂·4H₂O and the pollen germination percentage was nearly 74.46% and the average pollen tube length reached 258.84 μm after a 24 h incubation at 25 ℃.