In order to improve the rice blast resistance of ‘Yunzijing 41’and‘Yunzijing 43’,we transformed bivalent expression vector pCAMBIA1300-Pi-ta⁺-Bchi into rice calli using the agrobacterium- mediated method.Regeneration seedlings through tissue culture are tested through CPR assay,PCR and rice blast resistant assay method and a part of these are determined to be transgenic plants.This experiment laid the foundation for breeding the longer and higher blast resistance rice variety in future.Results show:(1)Resistant callus passed through differentiation and rooting cultivation,we received 137 T⁰ generation of rice seedlings,including 14 ‘Yunzijing 41’,82 ‘Zhonghua 11’,41 ‘Yunzijing 43’.(2)After CPR assay and PCR,the conversions of ‘Yunzijing 41’,‘Yunzijing 43’ and ‘Zhonghua 11’transgenic positive rice plants were 66%,43% and 55%,respectively.Transgenic plants were confirmed that two exogenous genes had been integrated into the rice genome.(3)After be identified by inoculation with rice blast strain 66 b,transgenic plants enhanced clearer resistance to rice blast than non-transgenic plants;at the same time,transgenic plants of Pi-ta⁺ gene and bean chitinase gene had more obvious ability of resistance to rice blast than transgenic plants of Pi-ta⁺ gene or bean chitinase gene.(4)CPR assay results existed in some false positives,PCR detection results are more reliable.But CPR assay is more convenient,faster and the results is more intuitive.This can be used for preliminary screening.The research indicates that transgenic plants of Pi-ta⁺ gene and bean chitinase gene had a higher ability of resistance to rice blast.