In order to construct prokaryotic expression vector and to study the recombinant gene expression in the host bacteria,we cloned complete gene of Triticum aestivum FeSOD ORF by the RT-PCR method followed by restriction enzymes cutting and connection.The induced time,IPTG concentration and temperature of prokaryotic expression were optimized using SDS-PAGE.The results showed that the full-length cDNA sequence of T.aestivum FeSOD ORF(600 bp) was cloned,then a recombinant of prokaryotic expression vector pET-SOD was constructed successfully.FeSOD protein in inclusion body can express effectively after the expression vector pET-FeSOD was transformed to Rosetta (DE3).The SDS-PAGE results indicated that pET-FeSOD fusion protein was expressed with molecular weight of 25.8 kD,with the pET-Duet1’s own induction produced 3.0 kD protein.The result is consistent with the 22.8 kD protein which encoded by FeSOD gene.The optimal expression condition was set as 0.5 mmol/L IPTG,induced temperature 37 ℃,and induced time 5 hours.These results are expected to lay a foundation for further studies on the properties and function of this gene.