The full length coding sequence without the TAA stop codon of LRR-RLKs gene (RLK6) of Arabidopsis thaliana was cloned by RT-PCR and fused with green fluorescent protein （GFP） gene to construct a plant expression vector.The stable Arabidopsis transformants were observed using confocal laser scanning microscope.The result indicated that RLK6-GFP fusion protein was localized in the plasma membranes.Consistent location was obtained by the experiment of RLK6-GFP transient expression in living Arabidopsis protoplasts.To visualize the temporal and spatial expression patterns RLK6 in plant tissue,a 2 063 bp promoter fragment of RLK6 was fused with the GUS gene to generate transgenic plants.GUS activity was detected in seedlings,roots and flowers,especially in anther,but hardly detected in stems,rosette leaves and dry seeds.Consistent result was obtained by RT-PCR analysis.Overall,these data implied that RLK6 might involve in the signaling pathway of flower organs development or related processes.