Morphology,cytology and STS,SSR markers were adopted to identify the dwarf line WB7-3 selected from the progenies of common wheat cv.7182×two-rowed barley.The results showed that WB7-3 grew evenly presenting favorable agronomic traits and its plant height was very low;the chromosome number of WB7-3 in root tip cells was 42 with 21 bivalents observed in most PMC at MⅠ,and no fluorescence signals appeared in genomic in situ hybridization (GISH) analysis using barley whole genome DNA;but special strip of barley emerged in WB7-3 at STS marker ABC454-one specific STS primer from barley.Meanwhile,the result of amplification using 210 SSR markers from wheat each chromosome was that all strips of wheat emerged in WB7-3 except four markers (Xgdm35,Xgdm5,Xgwm261 and Xgwm455) which from the short arm of 2D chromosome.It showed that WB7-3 was probably a 2DL/2HS small fragment translocation line.