A flowering-related MADS-box gene named NtAP1 (GenBank accession JN704304) was cloned from the flower bud of Narcissus tazetta var.chinensis using RT-PCR and RACE methods.The cDNA is 1 155 bp in length with an open reading frame 762 bp which is capable of encoding 253 amino acids.Phylogenetic analysis indicated that the gene belonged to the APl/AGL9 family AP1 lineage.RT-PCR revealed that the existence of NtAP1 was detectable in flowers,which the levels were the highest in pistil.This showed that the NtAP1 gene which cloned in this study did not play an important role in the formation process of double flowers of ‘Yulinglong’.
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YANG Chao, WU Jinghua, WU Shaohua. Clone and Expression of NtAP1 Honologous Gene from Narcissus tazetta var.chinensis[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(1):66-71