The RT-PCR combined with RACE method was used to clone the complete cDNA sequences and DNA sequences of plant-specific transcription factor DlGRAS4 and DlGRAS54 from embryogenic callus of Dimocarpus longan.The complete cDNA sequence of DlGRAS4 was 1 668 bp,encoding 431 amino acids,the complete cDNA sequence of DlGRAS54 was 2 113 bp,encoding 552 amino acids.DNA sequence analysis indicated that 5′-UTR of DlGRAS54 has a 1 533 bp intron.The sequences of both nucleotides and amino acids of the two members were high homologous with those of the known GRAS genes in other species.DlGRAS4 and DlGRAS54 located in plasma membrane;they had no signal peptide,had transmembrane structure and the GRAS protein domain.Anglicizing phylogenetic tree of GRAS in plants indicated that DlGRAS54 and AtPAT1,VvPAT1,PtPAT1,GmPAT1 belonged to the same branch;DlGRAS4 and AtSCL23,ZmSCL23 belonged to the same branch.Therefore,they putatively belonged to GRAS family.qPCR results indicated that DlGRAS4 showed approximately a “W” curve,it expressed at the highest levels in the globular embryos and cotyledonary embryos cultures.DlGRAS54 showed approximately an “M” curve,it expressed at the highest levels in the globular embryos and topedo embryos cultures,suggesting that DlGRAS4 and DlGRAS54 play a major role at the middle and late developmental stages of longan somatic embryogenesis.DlGRAS4 and DlGRAS54 showed up-regulated expression patterns under exogenous GA₃ treatment showed that they made the positive response with GA₃ treatment.