Cloning and Expression Analysis of Zinc Finger Protein Gene from Olimarabidopsis pumila
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    Abstract:

    Zinc finger protein plays important roles in plant stress tolerance.OpZFP,A zinc finger protein gene of Olimarabidopsis pumila,was isolated by homologous sequence cloning method.The open reading frame of OpZFP gene was 684 bp,encoding 227 amino acids which containing a typical Cys2/His2 zinc finger domain,an EAR-motif at its C-terminal region.Phylogenetic analysis revealed that OpZFP had a closer relationship with AtZFP1 and AlZFP1.A OpZFP promoter sequence covering 2 095 bp upstream of transcription start site was isolated from O.pumila,and showed only 84.4% similarity with that of AtZFP1 gene promoter.Several different cis-elements were found analyzed in two promoters.Expression analysis by semi-quantitative RT-PCR indicated that OpZFP displayed a much broader expression range in different tissues in O.pumila,with a maximum expression in root.OpZFP gene was induced by high salt,drought and low temperature,suggesting that the function of OpZFP might involve in some stress related signal pathways.

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GUO Danli, WANG Qiqi, WU Xiaoqing, FEI Chunyan, HUANG Xianzhong. Cloning and Expression Analysis of Zinc Finger Protein Gene from Olimarabidopsis pumila[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(2):243-250

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  • Online: March 14,2014
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