‘Qindao 2’,‘Xiangnuo Q33’ and ‘Qingxiangnuo’ as the donor parents of resistance gene,were selected to cross with ‘Hongxuenuo’ in order to improve the Hongxuenuos’ resistance to rice stripe virus;Meanwhile the polymorphism of three molecular markers consisted of ST10,H21 and STS11-43 were separately analyzed among the parents,and the polymorphic markers were detected in the different F₂ populations.The results showed that:(1)The polymorphism of molecular markers were different among the parents.The markers ST10 and STS11-43 were just detected between ‘Hongxuenuo’ and ‘Xiangnuo Q33’,and the marker H21 was detected between ‘Hongxuenuo’ and ‘Qindao 2’ or ‘Hongxuenuo’ and ‘Xiangnuo Q33’ or ‘Hongxuenuo’ and ‘Qingxiangnuo’.(2)From different kinds of molecular markers’ detection results to different hybrid combinations,it can be found that some individuals of F₂ generation have had the resistance gene,while few individuals had heterozygous resistance gene.(3)The performance of each plant in the field estimates that all of the individuals containing resistance gene are resistant to rice strip disease,which is accordant to the expected result of molecular detection;but to those plants containing heterozygous resistance genes,it still needs further 2~3 generations’ examination to get the pure resistance gene.In conclusion,this research demonstrated that the results of molecular assisted selection and the resistance of each plant in the field are consistent and those three different markers could be used to detect the resistance genes in the progenies of different hybrid combinations,which provided a viable method for modifying the Hongxuenuo’s resistance to rice stripe virus.