Structures of Organs and Determination of Camptothecin of Camptotheca acuminata Decne.
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    Abstract:

    The structures of different organs of Camptotheca acuminata Decne.were studied using anatomical methods and the dynamic changes of CPT contents of different organs were identified by HPLC.The result indicated that:(1)The structure of root longitudinal section of C.acuminata is the same as other dicotyledons,namely,it is composed of root cap,meristematic zone,elongation zone and maturation zone.The primary structures of root and stem are composed of epidermis,cortex and vascular cylinder.Osmiophilic cells are showed in cortexs and piths.In secondary growth,the cork cambium of root derives from pericycle,while the cork cambium of stem derives from cortex.With the secondary growth,the center of stem becomes hollow.The cross section of blade is composed of epidermis,mesophyll and vein,and osmiophilic cells are also found in the mesophyll.With scanning electron microscope and optics microscope observing,stomatal apparatus are only absent in adaxial epidermis of leaf and abnormity.The guard cells are nephroid,and subsidiary cells have not been observed.The leaf surface has three types of trichomes,one type is unicellular non-glandular which is distributed in the adaxial and abaxial epidermis of leaf.Other types are glandular hairs,in which one is global distributed in both adaxial and abaxial epidermis and the other is olivary distributed only in the abaxial epidermis.It was found that the younger the leaf is,the more the trichomes are distributed.(3)The HLPC results showed CPT contents in young leaf and seed is higher than that in xylem,the lowest in piths.CPT content of leaf gradually decreased with the leaf becoming mature.The content has differences between different parts in the same leaf.CPT contents have changed in the development of C.acuminata seedling.

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WANG Lingli, LIU Wenzhe. Structures of Organs and Determination of Camptothecin of Camptotheca acuminata Decne.[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(2):282-290

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  • Online: March 14,2014
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