In this experiment,the resistant plant,sensitive plant and the F₁ generation were used for identification of the molecular markers linked to salting tolerance gene of Fraxinus velutina by RAPD-BLA.The result indicated that 150 decamer random primers were used to amplify the DNA pools from the resistant plants and sensitive plants,only primer S20 could amplify a 592 bp specific fragment,and named S20-592.SCAR marker was developed from the RAPD marker S20-592 by cloning,sequencing and designing one pair of primers and the SCAR marker only presented in the individuals of the resistant plants.The fragment existed in the resistant plants and F₁ population,it was indicated that the SCAR primer can be used for rapid identification of salt tolerance F.velutina species.