In this study,the tissue culture seedlings of Vaccinium uliginosum Linn.were used as material,the EMS mutation system has been established,the alkali-resistance mutants were screened by using EMS and NaHCO₃ combinative stress method,and identified with some physiological indexes.The results showed that:(1)0.4% EMS immersion the stem 4 h was the suitable mutation concentration,survival rate could reach 46.7%;when 0.1% EMS was applied into the medium 3 days,survival rate could reach 50%;the suitable concentration of NaHCO₃ was 8 mmol·L^-1,after three times alternating culture,two candidate mutants have been screened;(2)Identification of physiological index showed the activities of SOD,POD and proline content which significantly higher than those in control,the content of MDA which significantly lower than those in control.The study showed the mutants have some ability in weak alkali-tolerance.