Mechanisms of Osmotic Adjustment of Themopsis lanceolata Test-tuble Plantlets in Adapting to Salt Stress
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    Abstract:

    To study the salt-tolerance mechanism of Thermopsis lanceolata,we determined the growth parameters,organic osmotic adjustment substance contents and inorganic ions such as Na+,K+ and Ca2+ cation contents of T.lanceolata test-tube plantlets which have been growing for 30 d under 0,0.2%,0.4%,0.6%,0.8%,1.0% NaCl and Na2SO4 Stress.The results showed:(1)With NaCl and Na2SO4 concentration increasing,the contents of free proline and soluble sugar in leaves of T.lanceolata test-tube plantlets increased significantly,but the increasing extent of free proline under NaCl stress was greater than that under the same Na2SO4 concentration stress,while the increasing extent of soluble sugar content under NaCl stress was smaller than that under the same Na2SO4 concentration stress;the soluble protein content increased first then decreased with the increasing of NaCl concentration while went up continuously with the increasing of Na2SO4 concentration.(2)Na+ content of T.lanceolata test-tube plantlets went up continuously with the increased of the NaCl and Na2SO4 concentrations and the Na+ contents of all treatments were significantly higher than those in the control.Ca2+ and K+ content in leaves presented a decreasing trend,which were significantly lower than that of the control,respectively.In roots,K+ content first increased and then declined.Compared with NaCl,Na2SO4 stress caused a lower increase of Na+ content and lower reduction of K+ and Ca2+ contents in leaves;Na+/K+and Na+/Ca2+ ratios increased with the increase of salt content;In leaves,Na+/K+and Na+/Ca2+ ratios under NaCl stress were higher than that under Na2SO4 stress,while Na+/K+and Na+/Ca2+ ratios in roots were lower than that under Na2SO4 stress.T.lanceolata test-tube plantlets under salt stress may adapt to salt environment depressing Na+ contents,increasing soluble sugar and soluble protein contents in leaves,higher K+ and Ca2+ contents and decreased Na+/K+and Na+/Ca2+ ratios in roots.In words,the adaptation of T.lanceolata to NaCl stress was smaller than that to Na2SO4 stress.

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ZHANG Chaoqiang, ZHENG Xiufang. Mechanisms of Osmotic Adjustment of Themopsis lanceolata Test-tuble Plantlets in Adapting to Salt Stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(5):963-969

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  • Online: May 30,2014
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