A synthetic wheat line CI184,susceptible wheat cultivar Mingxian169,the F₁ reciprocal crosses between CI184 and Mingxian169,F₂ and F_2:3 populations originated from the cross CI184/Mingxian169 were used as the plant materials to explore the genetic basis of the resistance in CI184.Simple sequence repeat(SSR),bulked segregant analysis(BSA) and the F₃ population’s disease data were used to map the resistance gene/locus in CI184.(1)The result of resistance test indicated that CI184 showed resistance to 30 isolates of Pst except V26 at seedling stage and showed resistance to Chinese prevailing isolates of Pst,including CYR32,CYR33,CY-Su4,CY-Su5,CY-Su7 and V26 at adult-stage.(2)The genetic analysis indicated that the stripe rust resistance in CI184 was mainly controlled by a recessive locus.(3)In this study,only one QTL was found on chromosome 1B,named Qyr.zz_1B,explaining 13.36% of phenotypic variance in Sichuan Province and 18.07% in Beijing.It was flanked by Xgwm18 and Xwmc626.(4)Three SSR markers linked with Qyr.zz_1B and one SSR marker for Yr15 could discriminate Qyr.zz_1B from the other resistant genes on 1B,such as Yr15,Yr24 and Yr26/YrCH42.This locus maybe potentially applied in wheat resistance breeding to stripe rust.