The gene MaFLS encoding the flavonol synthase(FLS) involved into flavonol synthesis was cloned from Muscari armeniacum.The full length cDNA of MaFLS was 1 076 bp,and the open reading frame length was 999 bp,which encoding a protein polypeptide of 332 amino acids with a predicted molecular weight of 38.51 kD and pI of 5.09.The putative MaFLS displayed identities to the FLSs of Arabidopsis thaliana and Sorghum bicolor of 60% and 83%,respectively,and contained several typical conserved elements found in the 2-oxoglutarate- and Fe(Ⅱ)-dependent dioxygenases(2-ODD).Quantitative real-time PCR analyzed the expression profiles of MaFLS in different tissues.The results demonstrated that MaFLS was constitutively expressed in roots,stems and leaves,with particularly high expression in flowers.The cDNA of MaFLS was then subcloned into pET-32a and introduced into BL21(DE3),and the recombinant plasmid was successfully expressed in the condition of 0.1 mmol·L^-1 IPTG at 25 ℃ for 20 h.The molecular weight was found to be about 38 kD by checking with SDS-PAGE,nearly equal to the predicated.