In this study,flagellin gene cloned from biocontrol strain Bs-916 (Bacillus subtilis) was introduced into rice by transgenic vector pCAMBIA1300,98 positive transgenic lines were obtained.PCR and RT-PCR test were carried out to detect the expression of target genes and 12 transgenic lines detected expression of fla gene.Subsequently,identification of disease resistance showed that three transgenic lines enhances resistance to Xanthomonas oryzae pv.oryzicola.It is clear from this research that fla gene can be used to control disease,provided a potential resistance gene for controlling bacterial leaf streak.These will shed light on their utility in crop improvement strategies.