In order to reveal the genetic diversity and relationship and provide a scientific basis for the conservation and utilization of peach accessions in Guizhou,we evaluated the genetic diversity of 71 accessions by SCoT markers.The results showed that:(1)16 SCoT primers generated 192 bands and 156 polymorphic markers.On average,the percentage of polymorphic bands was 81.25% and amplification site of each primer was 12.(2)The Nei’s genetic diversity index (H) was 0.265 0±0.186 1,the Shannon index of diversity (I) was 0.400 3±0.254 3.The Jaccard genetic similarity coefficients among the accessions were between 0.400 0 and 0.852 5.(3)Cluster analysis with UPGMA method showed that 71 peach accessions in Guizhou could be grouped into six groups at Jaccard coefficient 0.65,and 2 varieties of Baihuatao,2 varieties of Xuetao and 2 varieties of Qingtao were proved to be a homonym,respectively.The substantial genetic diversity was identified among the peach accessions in Guizhou,and the peach accessions of homonym could be differentiated by SCoT markers successfully.