Abstract:The formation of microspore,development of male gametophyte and germination rate of pollen grains in long-style flower of Luculia pinciana(Rubiaceae) were observed by traditional paraffin section technique and cytology compression method.The results showed:(1)Long-style flower of L.pinciana had five anthers,and the anther was tetralocular.(2)The anther wall was composed of one epidermis,one endothecium,two middle layers and one to three tapetums from outer to inner.The development type of anther wall was of basic type and the tapetum belonged to glandular type.(3)During meiosis of the microspore mother cells,cytokinesis was simultaneous type,and the tetrads were mostly tetrahedral,occasionally found isobilateral.Meiosis of microsporocyte was asynchronous between different anther chambers.(4)Mature pollen grains were two-cell type at dispersal stage.(5)Microsporogenesis and male gametophyte development of long-style flower of L.pinciana showed normally,and was of bisexual flower.(6)The artificial germination rate of pollen grains between on the stigma of long-styled and short-styled flowers was (91.8±1.6)% and (93.2±1.1)% respectively,and did not differ significantly(t=1.585,df=8,p=0.152).It was suggested that pollen grains of long-style flower germinated normally between on the two stigmas.