Neoalsomitra sarcophylla(Wall.) Hutch.is reported as a new record species of Cucurbitaceae from China.It is related to N.balansae(Gagnep.) Hutch.,but differs in its fruits 3-4 cm long,seeds 6-7 mm by 3-4 mm and shortly 2-horned at apex.In this study,matK,rbcL and psbA-trnH gene sequences of N.sarcophylla are acquired based on DNA barcoding technique and support the evidence of the species taxon after Blast.