The chromosome numbers and karyotypes of five species from genera Paraprenanthes Chang ex Shih,Pterocypsela Shih and Notoseris Shih(Compositae-Cichorium) were investigated with the conventional pressing plate method.The results show that their chromosome numbers all are 2n=18 and karyotype of Paraprenanthes diversifolia is reported for the first time.The karyotype formulas are expressed as 2n=8m+10sm(2SAT) for P.diversifolia,2n=4m+10sm+4st for Pterocypsela indica,2n=10m+8sm(2SC) for Notoseris macilenta,2n=10m(2SAT)+8sm for N.triflora,2n=12m(2SAT)+6sm for N.porphyrolepis.Paraprenanthes and Notoseris belong to Stebbins “2A” type and Pterocypsela belongs to Stebbins “3A” type.