In order to investigate the difference of quality and aroma composition of ‘Kyoho Grape’ between normal fruit under open cultivation and secondary fruit under protected cultivation,we used the solid phase micro-extraction(SPME) and gas chromatography-mass spectrometry(GC-MS) for determination.The result showed:(1)The single fruit weight,longitudinal diameter and transverse diameter of secondary fruits under protected cultivation were reduced by 8.23%,11.74% and 10.63% respectively,compared with ‘Kyoho Grape’ normal fruit with open cultivation.The total soluble sugar content increased by 8.95%,titratable acid content increased but the difference was not significant,fruit Vc content(1.47 mg·kg^-1) was significantly lower(2.00 mg·kg^-1) than that of control.The peel proanthocyanidins content were 3.37 times more than that of control and fruit color was significantly deeper than that of control.(2)Aldehydes were the richest compounds in secondary fruits of ‘Kyoho Grape’ with relative content of 53.55%,while esters were the richest in normal fruits with relative content of 61.36%.The relative content of ethyl acetate under open cultivation and the secondary fruit under protected cultivation were both higher,it showed that the two had obvious strawberry flavor.But aromatic compound of the highest relative content was 2-hexenal(41.14%) in secondary fruit.It was significantly higher than that of control(16.31%).The result showed that total soluble sugar content of secondary ‘Kyoho Grape’ was higher than that under open cultivation,but the fruit was smaller.Because 2-hexenal of grape berries had the very important influence on the aromatic components constitutes,it could change fruit flavor.Therefore,there were certain differences on fruit flavor between protected cultivation and open field cultivation of ‘Kyoho Grape’.