Cloning and Expression Analysis of TaMAPK2 Gene in Wheat
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    Abstract:

    MAPK protein kinase plays an important role in the response of abiotic stresses.In the present study,we isolated a MAPK gene designated TaMAPK2 from wheat.TaMAPK2 ORF is 1 110 bp,which encodes 369 amino acids.Comparison of amino acid sequences indicated that TaMAPK2 shared high similarity with MAPKs from other species,such as Aegilops tauschii (99%),Oryza sativa (94%) and Setaria italica (94%).Phylogenetic analysis showed that TaMAPK2 is closely related with OsMAPK2.Real-time quantitative polymerase chain reaction (QPCR) assay revealed that TaMAPK2 expression is induced after osmotic stress,low temperature,salt stress,ethylene and H2O2 treatments and inhibited after ABA treatment.These results suggested that TaMAPK2 participates in the response of abiotic stresses and related signaling molecules.

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HU Wei, YAN Yan, MA Zhanbing. Cloning and Expression Analysis of TaMAPK2 Gene in Wheat[J]. Acta Botanica Boreali-Occidentalia Sinica,2014,34(10):1962-1966

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  • Online: October 31,2014
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