To achieve the comprehensive utilization of walnut shell,we prepared pyroligneous acids from walnut shell of two species by pyrolyzing,and collected it at 90~550 ℃.Pyroligneous acids from walnut shell of Juglans regia L.(H) and Juglans sigillata Dode (T) were obtained after refinement.The basic properties of the pyroligneous acids were measured,and the chemical compositions were analyzed by Gas Chromatography-Mass Spectrometry(GC-MS).Moreover,antifungi activities of each pyroligneous acid were tested by using 7 phytopathogenic fungi.The results showed:(1)The basic properties and the chemical compositions of each pyroligneous acid were similar.(2)62 compounds were identified by GC-MS analysis,mainly organic acids,phenols,ketones and aldehydes.The content of organic acids was the highest,and followed was phenol compounds.Meanwhile,48 common compounds were obtained in the 2 pyroligneous acids,5 and 9 special compounds were obtained in H and T,respectively.(3)The 2 pyroligneous acids showed antifungi activities in different degrees,and they all showed the strongest inhibition on Rhizoctonia cerealis.The values of EC₅₀ of H and T on R.cerealis were 15.65 mg/mL and 15.23 mg/mL,respectively.It was considered that the chemical compositions of pyroligneous acids from walnut shell were complex.The pyroligneous acids showed high antifungi activities and could be exploited as a new resource.