Here a novel mutant zpr1 in Arabidopsis was screened and identified.In order to provide reference for crop architecture improvement,the phenotypic characteristics and molecular mechanism of the Arabidopsis mutant line zpr1 were studied.Mutant zpr1 is a recessive T-DNA insertion line and was investigated in phenotypic characteristics and genetic analysis.The gene was cloned and expressed in plant,as well as cis-acting elements were studied.(1)According to the observation of the phenotypic characters,comparing to the wild type Ws-2,the stem branch number of mutant line zpr1 was increased near terminal inflorescence.The stem leaves of mutant line zpr1 were lanceolate leaves different from the obovate leaves of wild-type.The other phenotypic characters such as the height of main inflorescence,plant height,branch height,and branch length in mutant zpr1 were higher than those in wild type,and the branch numbers were also higher.(2)Plasmid rescue and IPCR identified the position of mutant gene was 426 bp upstream of the start codon of the Arabidopsis gene ZPR1 and showed that the insertion of T-DNA fragment in the promoter region of ZPR1 abolished its expression.(3)Analysis of the cis-acting element of transcriptional regulatory region identified multiple regulatory elements which had relation to plant hormones and photoperiod.(4)The study on protein subcellular localization showed that ZPR1 protein mainly localized in cell membrane,occasionally localized in parts of cell membrane,cytoplasm and the nucleus.It revealed that the expression of ZPR1 has regulatory effects in the plant architecture development and the expression level will regulate the hormone sensitivity and light sensitivity.Eventually,this gene leads to the formation of plant type.