Acyl-CoA-binding protein(ACBP) has been proposed to play a pivotal role in the intracellular trafficking and the utilization of long-chain fatty acyl-CoA esters.The objective of this study is to demonstrate the structure and function of the JcACBP.A complete CDS sequence of Acyl-CoA-binding protein gene(JcACBP) was cloned from the Jatropha curcas L.We analyzed the sequence and structure characteristics of its encoded protein,and its expression patterns in the different organism and the development of fruit by quantity RT-PCR.The complete CDS sequence is 279 bp,and encoded 92 amino acid residues with a putative molecular mass of 10.30 kD.JcACBP contained one conserved domain-ACBP superfamily.Evolutionary relationships analysis revealed a closer genetic relationship between the J.curcas L.JcACBP gene and that of Vernicia fordii(96%).JcACBP expression significantly in different organism and had the highest transcript profile in the fruit;the expression level of JcACBP had the same trends with the lipid accumulation in the fruit.