To map the male-sterile gene in grape,we used a ‘WS’ population including 88 individuals derived from the selfing of ‘Wink’(Vitis vinifera L.) as materials.The bulked segregate analysis(BSA) technology was conducted to screen the polymorphism between the fertile and sterile pools by SSR technologies in order to locate the gene and carry out bioinformatics analysis.Two SSR markers of VVMD34 and VVIB23 were linked with VvMs1.They located on either sides with genetic distances of 3.5 cM and 1.9 cM,respectively.The physical distance between VVMD34 and VVIB23 is 1 134 kb based on the whole genome sequence of grape,and there are 111 candidate genes in this region.These results will benefit fine-mapping of male-sterile gene and marker-assisted selection(MAS) in grape breeding program.