In order to screening candidate resistance related genes against Lily Fusarium Wilt,we constructed a suppression subtractive hybridization(SSH) cDNA library from leaves of tissue-culture seedling of Lilium sargentiae Wilson induced by Fusarium oxysporum f.sp.lilii.300 clones of the forward SSH library were picked out randomly and sequenced,and then 280 qualified sequence tags(ESTs) were obtained.After functional annotation,168 ESTs were found putatively encoding functionally known protein.The function-known ESTs related to signal transduction,protein synthesis and transporters,disease resistance and defense,energy metabolism and transcription,etc.Among them,31 ESTs were the defense response genes,and 8 defense response ESTs(Catalase,ABC transporter,Kunitz trypsin inhibitor 4,Serine-glyoxylate aminotransferase,Polyubiquitin,lipoxygenase I,Serine/threonine-protein kinase,Arabidopsis thaliana) were analyzed by the semi-quantitative RT-PCR,the results showed they were upregulated by F.oxysporum f.sp.lilii and supposed to involved in the disease resistance reaction for Lily Fusarium Wilt.