The sequence-related amplified polymorphism(SRAP) were used to analyze 80 Ziziphus jujuba Mill.hybrid individuals and their parental lines for detecting true or false hybrids and their genetic variation.Fifteen pairs of primers that generated clear,reproducible and polymorphic bands were selected from 88 pairs of SRAP primers by constructing a gene pool.The results showed that:(1)There are 95 polymorphic bands produced by fifteen primer pairs and the average polymorphic bands was 6.3 per primer pair with a high rate of polymorphism.(2)44 truehybrids were identified among 80 hybrids with paternal characteristics.Diversity and UPGMA cluster analysis showed significant genetic variation of hybrids.The genetic similarity coefficients between individual and parental are from 0.55 to 0.98.The results provided an important molecular data for crossbreeding in Ziziphus jujuba Mill..