Variations of endogenous hormone content in seeds of Cornus macrophylla during cold stratification were studied with enzyme linked immunosorbent assay(ELSA),the relationship between endogenous hormone content and seed dormancy and germination was analyzed.The result showed that:(1)IAA content in seeds dropped in the early phase of cold stratification,but that rebounded after brief continuant time,and the variation of IAA/ABA was the same.ABA content in seeds was high before cold stratification,but as the extension of treatment time that tended to fall.Both GA_1/3 content and GA_1/3/ABA were gradually increasing with the delay of the low-temperature stratification time.Variations of both ZRs and iPAs contents showed a smooth and steady situation,they trended to increasing despite some fluctuations.(2)The seeding experiment showed that the time of low-temperature stratification has important effects on seeds germination.Germination rate and germination potential of seeds without the low-temperature stratification treatment and these of processing time was less than 90 d were zero,the two index rose as the extension of stratification time.Thus effect of stratification time on seed germination of C.macrophylla seeds was obvious.(3)The results of correlation analysis between endogenous hormone and germination indexes showed that there were significantly positive correlation(P<0.05) between GA_1/3 and germination rate,germination potential,and the correlation coefficients were 0.688 and 0.662,respectively.Increase of GA_1/3/ABA could promote the releasing dormancy and germination of C.macrophylla seeds.