To further optimize Camellia oleifera regeneration system,and to understand about the physiological characteristics of different age of explants,we chose ‘Changlin 53’ young embryo,made multi-factor and multi-level orthogonal experiment.The results showed that:The fruit rapid growth in July and August,the content of soluble protein,total soluble sugar and sucrose slowly increased in this time.The content of ABA(110.13 ng·g^-1),the ratio ABA and ZR(13.47) reached the highest in immature embryo.Effect of the inducing 2,4-D on somatic embryo was the largest,followed by was embryo age.The optimal formula of ‘Changlin 53’ somatic embryo induction was took in MS added 0.5 mg·L^-1 2,4-D,0.5 mg·L^-1 KT,0.05 mg·L^-1 TDZ and 500 mg·L^-1 Gly,then took the immature embryo in rapid expanding period as explants.Great influence of the IAA on embryoids germination,the optimal formula of somatic embryo germination and plantlet growth was in MS added 2 mg·L^-1 IAA,0.5 mg·L^-1 6-BA,0.5 mg·L^-1 TDZ and 500 mg·L^-1 CH.