The plant growth,selenium(Se) uptake and transportation in plant organs of flue-cured tobacco ‘Yunyan 87’ exposed to soil with different Se concentration(0.30,0.45,1.00,1.75 mg·kg^-1) were studied by a pot experiment in tobacco growing areas of Chizhou,Anhui province.Results showed that:(1)The appropriate amount of Se in soils(≤1.00 mg·kg^-1) stimulated plant growth but high concentration Se(≥1.75 mg·kg^-1) inhibited plant growth.The Se concentration in the roots,shoots,and leaves of the tobacco plants significantly increased with the increase of Se in soils.(2)The pattern of total Se concentration in the tobacco plant tissues followed the order:root>leaf>shoot.The Se concentration in root was 2～3 times of the corresponding leaf,and leaf was 3～4 times of the stem.(3)When the Se concentration of soil increased from 0.30 to 1.75 mg·kg^-1,the root absorption index(RAI) of Se significantly reduced from 1.08 to 0.36,but the secondary transport index(STI) of Se significantly increased from 2.84 to 4.03.These suggested that the Se absorption efficiency of root was decreased,while the transport and accumulation of Se in leaf were increased with increasing Se concentration in soil.(4)The plants accumulated the maximum Se(72 μg) when the amount of Se in soils was 1.00 mg·kg^-1.Therefore,we could produce Se-enriched tobacco leaf under soil Se concentration within the range of 0.45 to 1.00 mg·kg^-1 without spraying Se fertilizer to reduce the cost and avoid soil and water pollution caused by exogenous Se.