LIM domain proteins are important members of transcription factors in eukaryotes,which play important roles in the regulation of growth and development including gene transcription,organizing cytoskeleton and signal transduction.The full-length cDNA of GhLIMa was obtained by electronic cloning and RT-PCR method(The accession No.of Genbank is DQ672342) from salt resistant cotton materials ‘H15’.It includes 627 bp open reading frame and encodes a protein about 23.2 kD which is composed of 208 amino acids.Bioinformatics analysis revealed that GhLIMa contains two full LIM domain structures.The amino acid sequences of LIM from cotton and other species were selected and analyzed by multi-sequence alignment.The results showed that they have high similarity (>78%) with those of Ricinus communis,Theobroma cacao,Populus trichocarpa.The expression of GhLIMa from leaves and roots of salt tolerance and salt sensitive cotton material was detected by quantitative real-time RT-PCR.The results showed that GhLIMa gene can be induced by salt in leaves and roots.It suggested that GhLIMa plays an important role in cotton response to salt stress.