A ethylene-responsive factor gene,named NhccERF1,was isolated from bud of non-heading Chinese cabbage(Brassica campestris ssp.chinensis),named as “Subai 2”,which has stamens mutation and its maintainer lines by cDNA-AFLP technique.(1)Sequence analysis showed that the full length sequence of NhccERF1 gene was 807 bp,encoded 268 amino acids residues.(2)RT-PCR results showed that NhccERF1 gene have a highly expression in stamen,petal,carpel in mutant than in maintainer lines.(3)Quantitative RT-PCR analysis indicated that,external ethylene increased the gene expression,while external AgNO₃ inhibited its expression.This results suggested that the gene might involved in stamen mutant.