In order to study the function of AtTIP5;1 gene,we employed Saintpaulia ionantha leaves as receptor materials and optimized the genetic transformation conditions.The transgenic plants were exposed to high concentration of boric acid and the resistant ability was evaluated.The results showed:(1)The receptor leaves pre-cultured for 2 d,infected for 15 min,co-cultured of 2 d with 100 μmol/L AS,and selected with 40 mg/L hygromycin were the optimum transformation conditions.(2)After PCR and RT-PCR screening,17 transformed plants containing AtTIP5;1 transgene were obtained.(3)Transformed and control plants were treated by high concentration boric acid (3 mmol/L) and physiological indexes of these plants were evaluated.The results indicated that the AtTIP5;1 expression could be induced by boron.Transgenic plants showed a lower degree of plant tissue browning,leaves curling,and a higher rate of dry matter,soluble sugar and soluble protein content.Meanwhile,the increase of POD and SOD activities were weaken.Our results demonstrate that AtTIP5;1 trasformation can improve the tolerance of S.ionantha to the high boron stress.Our study is helpful for further investigation on AtTIP5;1 gene function and gene engineering in breeding of S.ionantha.