In higher plant,ICE1 involved in the response to abiotic and biotic stresses by regulating the expression of CBF genes.The cDNA sequence of DcICE1 transcription factor was cloned from carrot cultivars ‘Heitianwucun’ and ‘Junchuanhong’ using RT-PCR method,respectively.In order to analyze the response of DcICE1 gene to abiotic stress,the expression profiles of DcICE1 gene in four different stresses(4 ℃,38 ℃,0.2 mol·L^-1 NaCl,200 g·L^-1 PEG) were detected by quantitative real-time PCR.Sequence analysis indicated that the DcICE1 gene contained 1 458 bp,encoding 485 amino acids.There were two nucleotide sites and two amino acid residue differences between two cultivars(G/A in site 139 and A/G in site 475;E/K in site 47,N/D in 159).The different amino acid residues may be involved into the different response in two carrot cultivars encountered the abiotic stresses.The DcICE1 transcription factor has a conserved domain.Quantitative real-time PCR detected the expression profiles of DcICE1 gene under abiotic stresses.The results showed the expression of DcICE1 gene significantly increased when exposed to 4 ℃,while the expression profiles of DcICE1 gene was not obvious after treated by salt and PEG in the two carrot cultivars.