An expressed sequence tag(GenBank accession number:JZ151854) from the 5′ end which was highly similar to NAC transcription factor AtNAC026 of Arabidopsis thalania was obtained,through sequencing of clones randomly selected from the previously constructed cDNA library of Olimarabidopsis pumila leaves.This clone was sequenced from the 3′ end,and a full-length cDNA of 1 327 bp was obtained after sequence assembly.Its open reading frame (ORF) was 906 bp,and encodes 301 amino acids.The primers were designed according to the ORF and this gene was cloned from O.pumila by RT-PCR technique,which was designated as OpNAC026 (GenBank accession number:KM457621).Physicochemical property analysis showed that OpNAC026 is a hydrophilic protein with a conserved domain at its N-terminal region,and OpNAC026 has no transmembrane domain,indicating that it is not a transmembrane protein.The secondary structural analysis showed that OpNAC026 contains 54 α-helixes and 12 β-turns.The phylogenetic analysis revealed that OpNAC026 showed the closest genetic kinship with A.thaliana AtNAC026 and AtNAM,indicating that they belonged to the same evolutionary branch.Quantitative real-time PCR (qRT-PCR) revealed that OpNAC026 displayed a much broader expression range at O.pumila different tissues,with a maximum expression in leaves.The transcriptional level of OpNAC026 was up-regulated as 24 h of NaCl treatment,12 h of 20 % PEG-6000 treatment,6 h of ABA treatment and 8 h of 4 ℃ treatment,respectively.Our research indicated that OpNAC026 might control the mechanism of resistance in O.pumila.