With cherry tomato cultivar ‘Qianxi’ as test material,the experiment was carried out with vinegar residue substrate to investigate the effects of different concentrations(0.05,0.10,0.20,0.40 mg·L^-1) of 24-epibrassinolide on chlorophyll content,gas exchange parameters,chlorophyll fluorescence parameters,yield and fruit quality of cherry plants.The results showed that:(1)The chlorophyll content,net photosynthetic rate(P_n) and transpiration rate(T_r) were significantly enhanced by 0.05,0.10 and 0.20 mg·L^-1 EBR.Chlorophyll fluorescence parameters such as Φ_PSⅡ,F_v′/F_m′,q_P and ETR were significantly increased at the concentration of 0.10 mg·L^-1 EBR,the activities of carbonic anhydrase and Rubisco were also increased by EBR.(2)0.10 mg·L^-1 EBR significantly increased levels of lycopene and beta-carotene,soluble solids,soluble sugar and vitamin C in cherry fruit,and also EBR significantly increased fruit weight and yield by 17.5% and 33.6% as compared to the control,respectively.These results indicated that EBR could improve the yield and quality of tomato fruits by strengthening the photosynthetic enzymatic activity and promoting growth,and the treated concentration of 0.10 mg·L^-1 EBR showed the best effects on growth in cherry tomato plants.