Using Arabidopsis thaliana(Columbia ecotype) as material,we cloned calcium-dependent protein kinase 1(AtCDPK1) gene with PCR and recombinant DNA technologies.The full length of the gene is 2 269 bp,and the sequence of bases was entirely consistent with that of the known gene At1g18890.We constructed the plant expression vector pCHF3-CDPK1 of AtCDPK1 gene,and transformed pCHF3-CDPK1 into virus-free miniature of potato cultivar ‘Favorita’ using Agrobacterium-mediated method.After plant selection and regeneration,a total of 50 regenerated plants with resistance were obtained successfully.The PCR detection showed that a total of 36 plants contained AtCDPK1 gene,and the RT-PCR analysis proved that the AtCDPK1 gene in transgenic potato plants could conduct normal transcription and expression.This research lays the foundation for further function analysis of AtCDPK1 gene in transgenic potato and breeding new drought-resistant transgenic potato.