Separation and Functional Analysis of the Promoter of PpAGO7 Gene from Physcomitrella patens
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    Abstract:

    The AGO7 was found in many plants and was predicted to play a role in the process during growth and development of leaves in higher plants.However,it was not reported in bryophytes which was one of the oldest and the only one which had no vascular bundle in higher plants.By comparison to the amino acid sequence of OsAGO7 in rice with BLAST tool,we obtained the AGO7 protein code gene in Physcomitrella patens named PpAGO7.We also cloned the promoter sequence of PpAGO7 and analyzed its sequence characters by using online software Plant CARE and PLACE.Then the promoter analysis vector pPpAGO7-GUS was built and transferred into Arabidopsis to detect the initiation activity.The result indicated that:(1)The promoter sequence contained large amount of cis-acting elements related to the photo reaction and a plurality of elements related to the meristem and defense and oppression related elements.(2)According to the staining result of the T2 transgenic plants,GUS staining was found in different tissues and during different growth periods.Moreover,the staining color was much deeper in the root,leaf tip,anther of stamen,stigma of pistil and seeds than in other tissues.(3)The GUS enzymatic activity of the transgenic Arabidopsis under the light intensity condition 1 000 lx or 4 000 lx was lower than that under the light intensity condition 7 000 lx or 10 000 lx.All results indicted that the cloned PpAGO7 promoter had constitutively promoting activity and was influenced by light intensity.

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GAO Yuntong, PAN Shenyuan, WANG Aimin. Separation and Functional Analysis of the Promoter of PpAGO7 Gene from Physcomitrella patens[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(3):465-471

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  • Online: April 13,2015
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