Taking the aseptic seedling of ‘Shepody’ potato which bring virus as materials,38 ℃/4 h heat treatment for 4 weeks,stripping with one leaf primordia of potato meristem,inoculated to 24 kinds of MS solid medium add to different hormone combinations,statistical the callus induction rate of meristem and differentiation seedling rate after culturing 30 days by 22 ℃/16 h;RT-PCR detected the virus-free rate of meristem regeneration of three kinds of potato viruses(PVX,PVY,PLRV) and potato spindle tuber viroid(PSTVd).The results showed that the best meristem differentiation medium was MS+1.0 mg/L ZT+0.2 mg/L NAA+2.0 mg/L GA₃,the callus induction rate was 76.25%,differentiation seedling rate was 26.25%;the rate of detoxification of regenerated plantlets three potato virus PVX,PVY and PLRV were 69.4%,91.7% and 100%,respectively.The virus-free rate of potato spindle tuber viroid was 8.3% and the secondary shoot tip virus-free rate was 20.8%.