The MYB transcription factor is one of the largest gene families in plant,involving in various physiological and biochemical processes.In this study,one new MYB gene TaMYB3-4D was isolated from the wheat cultivar ‘Gaoyuan 115’.TaMYB3-4D was close to the genes regulating anthocyanins biosynthesis in phylogenetic tree.TaMYB3-4D possessed only one intron,while the other MYB genes regulating anthocyanins biosynthesis had two introns.The deduced amino acid of TaMYB3-4D contained two consecutive MYB domains,the typical characteristic of the R2R3-MYB transcription factor.Transient expression experiments showed that TaMYB3-4D induced the white coleoptile cells of wheat cultivar Chinese Spring (CS) to synthesize anthocyanins with the help of bHLH transcription factor ZmR in light.Meanwhile,the RT-PCR analysis showed that TaMYB3-4D gene only expressed in purple seeds and red coleoptiles of ‘Gaoyuan 115’,but not in root,stems and leaves.All of these results suggested that TaMYB3-4D was a R2R3-MYB transcription factor regulating anthocyanins biosynthesis in wheat.