To study the function of somatic embryogenesis receptor-like kinase gene (SERK) in Anthurium andraeanum’ s somatic embryogenesis,we isolated a novel AaSERK gene from the embryogenic callus of A.andraeanum by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE).The expression levels of AaSERK were analyzed by Real time-PCR.The results showed that:(1)The full-length cDNA of AaSERK was 1 949 bp with an open reading frame of 1 866 bp encoding a protein of 622 amino acid residues.(2)It was speculated that the AaSERK was a typical SERK gene with one signal peptide,one leucine zipper domain,five Leu-rich repeat(LRR) domains,one SPP motif,one transmembrane domain,the kinase domain with eleven subdomains,and one C-terminal domain.DNAMAN analysis showed that the putative AaSERK amino acid sequence had 65%-89% identity with those of the other plants.(3)In the different stages of somatic embryogenesis in vitro,the expression of AaSERK was weak or little in the inducing stage and the developmental stage,but was the highest in the embryogenic callus on the 30th day of the subculture stage.It was speculated that the AaSERK might be one of the marker genes of somatic embryogenesis in A.andraeanum.