For preliminary study the function of vacuole H⁺-ATPase subunit c gene (VHA-c1) in plant growth and signal transduction,we constructed an over-expression vector of VHA-c1 in Arabidopsis thaliana and introduced it into wild-type A.thaliana.The expression levels of VHA-c1 were analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR),then the VHA-c1 transgenic homozygotes were treated by dark condition,ABA or sugar.The results showed that:(1)7 lines of VHA-c1 tansgenic homozygotes were obtained,the corresponding transcript level of them increased,indicating that the overexpression constructs are effective.(2)When grown under dark condition,the root length in 6 VHA-c1 tansgenic homozygotes lines were reduced.(3)With treatment by ABA,4 lines reduced the sensitivity in taproot growth and the extent of cotyledon unfolding;7 lines reduced the sensitivity in seed germination.(4)5 lines and 4 lines also reduced the sensitivity to inhibition on seed germination by glucose and sucrose,respective.This indicates that the vacuolar VHA-c1 might be involved in the signalling pathway mediated by ABA and sugar,and possible effect on cell expension.