Phalaenopsis ‘Taiwan’s Golden’(seedling age 15 months) were used as the study materials.Its leaves were sprayed with 200 μmol·L^-1 SNP(NO donor),and then plants were treated under 12 ℃/7 ℃(day/night).The electrolyte leakage rate,MDA content,pH,osmotic adjustment substances contents and protective enzymes were determined on five days and ten days after cold treatment.The results showed that the SNP treatment could restrain the increase of pH,electrolyte leakage rate and MDA content effectively,increase the contents of soluble sugar,soluble protein and proline and delay the decrease of SOD,POD,CAT and APX activities significantly,enhance the activities of PPO and PAL.The above results indicated the SNP treatment could reduce the damage under low temperature,improve the ability of resistance to low temperature stress in Phalaenopsis plants through protecting the cell membrane system,increasing the osmotic adjustment substance contents and improving the activities of protective enzymes.